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recombinant human vegf rhvegf  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human vegf rhvegf
    Recombinant Human Vegf Rhvegf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+vegf+rhvegf/Human+Galectin-3BP%2FMAC-2BP+Antibody/pmc13112773-316-24-31
    Average 93 stars, based on 25 article reviews
    recombinant human vegf rhvegf - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Immune cells regulate VEGF signalling via release of VEGF and antagonistic soluble VEGF receptor‐1
    Article Snippet: .. For evaluation of cell culture supernatants, interference with VEGF detection, the supernatants and the calibrator diluent were each spiked with 400 pg/ml recombinant human VEGF (rhVEGF) (R&D Systems) and incubated for 30 min at room temperature before determination of VEGF concentration by ELISA. ..

    Recombinant:

    Article Title: Immune cells regulate VEGF signalling via release of VEGF and antagonistic soluble VEGF receptor‐1
    Article Snippet: .. For evaluation of cell culture supernatants, interference with VEGF detection, the supernatants and the calibrator diluent were each spiked with 400 pg/ml recombinant human VEGF (rhVEGF) (R&D Systems) and incubated for 30 min at room temperature before determination of VEGF concentration by ELISA. ..

    Article Title: VEGF Secreted by Hypoxic Müller Cells Induces MMP-2 Expression and Activity in Endothelial Cells to Promote Retinal Neovascularization in Proliferative Diabetic Retinopathy
    Article Snippet: .. Recombinant mouse VEGF (rmVEGF), recombinant human VEGF (rhVEGF), MMP-2, and VEGF ELISA kits were obtained from R&D Systems. .. Predesigned control (scrambled) and VEGF small interfering RNA sequences were obtained from Qiagen.

    Article Title: Vascular Endothelial Growth Factor Inhibits Bone Morphogenetic Protein 2 Expression in Rat Mesenchymal Stem Cells
    Article Snippet: .. MSCs were transfected with 100 PFU/cell of the BMP2 adenovirus and then exposed to the medium supplemented to a final concentration of 10 or 50 ng/mL recombinant human VEGF (rhVEGF) (R&D Systems) for 48 h. BMP2 production was then determined through the ELISA measurement (R&D Systems Quanitkine Kit) as described above. ..

    Article Title: Curcumin inhibits hepatocellular carcinoma growth by targeting VEGF expression
    Article Snippet: The polymer horseradish peroxidase (HRP) detection systems, used for immunohistochemistry and western blotting, were purchased from Zhongshan Golden Bridge Biotechnology Co., Ltd (Beijing, China) and Cellular Signaling Technology (Danvers, MA). .. Recombinant human VEGF (rhVEGF) was from R&D Systems, Inc. (Minneapolis, MN, USA). .. The H22 HCC cell line was purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences, Shanghai Institute of Cell Biology, Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum (Hyclone; GE Healthcare Life Sciences, Logan, UT, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin in a humidified incubator at 37°C with 5% CO 2 .

    Article Title: Non-clinical pharmacology and toxicology studies of bevacizumab biosimilar LY01008.
    Article Snippet: LY01008 was a biosimilar of Avastin® developed by Shandong Boan Biotechnology.. To support the clinical trial and marketing application of LY01008 as a biosimilar, a series of non-clinical pharmacodynamics (PD), pharmacokinetics (PK), and toxicological studies have been conducted.. The PD study results showed that LY01008 had similar pharmacodynamic effects with Avastin in VEGF (vascular endothelial growth factor) binding activity, inhibitory effect on angiogenesis and vascular permeability, and anti-tumor activities in nude mouse models alone or combined with chemotherapeutic agents.

    Article Title: Isolinderalactone suppresses human glioblastoma growth and angiogenic activity in 3D microfluidic chip and in vivo mouse models.
    Article Snippet: Glioblastoma multiforme (GBM) is a lethal and highly vascular type of brain tumor.. We previously reported that isolinderalactone enhances GBM apoptosis in vitro and in vivo, but its role in tumor angiogenesis is unknown.. Here, we investigated the anti-angiogenic activity of isolinderalactone and its mechanisms.

    Article Title: The Galectin-3-binding protein promotes angiogenesis in pancreatic cancer via simultaneous upregulation of VEGFA and direct HUVEC activation mediated by and VAMP5-STAT3
    Article Snippet: .. Lapatinib (S2111, Selleckchem), MK-2206 (S1078, Selleckchem), and S3I-201 (S1155, Selleckchem) were pre-treated on HUVECs 1 h before recombinant human Gal-3BP (rhGal-3BP) stimulation. rhGal-3BP and recombinant human VEGF (rhVEGF) were obtained from R&D systems (rhGal-3BP; AF2226-GAB, rhVEGF;293-VE). ..

    Incubation:

    Article Title: Immune cells regulate VEGF signalling via release of VEGF and antagonistic soluble VEGF receptor‐1
    Article Snippet: .. For evaluation of cell culture supernatants, interference with VEGF detection, the supernatants and the calibrator diluent were each spiked with 400 pg/ml recombinant human VEGF (rhVEGF) (R&D Systems) and incubated for 30 min at room temperature before determination of VEGF concentration by ELISA. ..

    Concentration Assay:

    Article Title: Immune cells regulate VEGF signalling via release of VEGF and antagonistic soluble VEGF receptor‐1
    Article Snippet: .. For evaluation of cell culture supernatants, interference with VEGF detection, the supernatants and the calibrator diluent were each spiked with 400 pg/ml recombinant human VEGF (rhVEGF) (R&D Systems) and incubated for 30 min at room temperature before determination of VEGF concentration by ELISA. ..

    Article Title: Vascular Endothelial Growth Factor Inhibits Bone Morphogenetic Protein 2 Expression in Rat Mesenchymal Stem Cells
    Article Snippet: .. MSCs were transfected with 100 PFU/cell of the BMP2 adenovirus and then exposed to the medium supplemented to a final concentration of 10 or 50 ng/mL recombinant human VEGF (rhVEGF) (R&D Systems) for 48 h. BMP2 production was then determined through the ELISA measurement (R&D Systems Quanitkine Kit) as described above. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Immune cells regulate VEGF signalling via release of VEGF and antagonistic soluble VEGF receptor‐1
    Article Snippet: .. For evaluation of cell culture supernatants, interference with VEGF detection, the supernatants and the calibrator diluent were each spiked with 400 pg/ml recombinant human VEGF (rhVEGF) (R&D Systems) and incubated for 30 min at room temperature before determination of VEGF concentration by ELISA. ..

    Article Title: VEGF Secreted by Hypoxic Müller Cells Induces MMP-2 Expression and Activity in Endothelial Cells to Promote Retinal Neovascularization in Proliferative Diabetic Retinopathy
    Article Snippet: .. Recombinant mouse VEGF (rmVEGF), recombinant human VEGF (rhVEGF), MMP-2, and VEGF ELISA kits were obtained from R&D Systems. .. Predesigned control (scrambled) and VEGF small interfering RNA sequences were obtained from Qiagen.

    Article Title: Vascular Endothelial Growth Factor Inhibits Bone Morphogenetic Protein 2 Expression in Rat Mesenchymal Stem Cells
    Article Snippet: .. MSCs were transfected with 100 PFU/cell of the BMP2 adenovirus and then exposed to the medium supplemented to a final concentration of 10 or 50 ng/mL recombinant human VEGF (rhVEGF) (R&D Systems) for 48 h. BMP2 production was then determined through the ELISA measurement (R&D Systems Quanitkine Kit) as described above. ..

    Transfection:

    Article Title: Vascular Endothelial Growth Factor Inhibits Bone Morphogenetic Protein 2 Expression in Rat Mesenchymal Stem Cells
    Article Snippet: .. MSCs were transfected with 100 PFU/cell of the BMP2 adenovirus and then exposed to the medium supplemented to a final concentration of 10 or 50 ng/mL recombinant human VEGF (rhVEGF) (R&D Systems) for 48 h. BMP2 production was then determined through the ELISA measurement (R&D Systems Quanitkine Kit) as described above. ..



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    Expression of CAP markers and VEGF-A <t>isoforms</t> during human prenatal lung development. RT-qPCR analysis of the expression of universal capillary marker CA4 and CAP2 specific markers ( SOSTDC1 , HPGD, EDNRB, CA4, APLN, and TBX2 ) (A) and of total VEGF-A ( VEGF-Atot ) as well as each VEGF-A isoform ( VEGF-A121 , VEGF-A145 , VEGF-A165 , VEGF-A189 ) (B) in native human prenatal lungs aged from the late pseudoglandular to the early canalicular stages of development (week 10–20 of GA). Results are shown as a dot plot with mean ± SEM, n = 8–10, * p < 0.05, ** p < 0.01, *** p < 0.001. (C) Visual summary of the VEGF-A isoforms and CAP2 markers expression during human prenatal lung development. Image created by Biorender.com .
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    Expression of CAP markers and VEGF-A <t>isoforms</t> during human prenatal lung development. RT-qPCR analysis of the expression of universal capillary marker CA4 and CAP2 specific markers ( SOSTDC1 , HPGD, EDNRB, CA4, APLN, and TBX2 ) (A) and of total VEGF-A ( VEGF-Atot ) as well as each VEGF-A isoform ( VEGF-A121 , VEGF-A145 , VEGF-A165 , VEGF-A189 ) (B) in native human prenatal lungs aged from the late pseudoglandular to the early canalicular stages of development (week 10–20 of GA). Results are shown as a dot plot with mean ± SEM, n = 8–10, * p < 0.05, ** p < 0.01, *** p < 0.001. (C) Visual summary of the VEGF-A isoforms and CAP2 markers expression during human prenatal lung development. Image created by Biorender.com .
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    Expression of CAP markers and VEGF-A <t>isoforms</t> during human prenatal lung development. RT-qPCR analysis of the expression of universal capillary marker CA4 and CAP2 specific markers ( SOSTDC1 , HPGD, EDNRB, CA4, APLN, and TBX2 ) (A) and of total VEGF-A ( VEGF-Atot ) as well as each VEGF-A isoform ( VEGF-A121 , VEGF-A145 , VEGF-A165 , VEGF-A189 ) (B) in native human prenatal lungs aged from the late pseudoglandular to the early canalicular stages of development (week 10–20 of GA). Results are shown as a dot plot with mean ± SEM, n = 8–10, * p < 0.05, ** p < 0.01, *** p < 0.001. (C) Visual summary of the VEGF-A isoforms and CAP2 markers expression during human prenatal lung development. Image created by Biorender.com .
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    Expression of CAP markers and VEGF-A <t>isoforms</t> during human prenatal lung development. RT-qPCR analysis of the expression of universal capillary marker CA4 and CAP2 specific markers ( SOSTDC1 , HPGD, EDNRB, CA4, APLN, and TBX2 ) (A) and of total VEGF-A ( VEGF-Atot ) as well as each VEGF-A isoform ( VEGF-A121 , VEGF-A145 , VEGF-A165 , VEGF-A189 ) (B) in native human prenatal lungs aged from the late pseudoglandular to the early canalicular stages of development (week 10–20 of GA). Results are shown as a dot plot with mean ± SEM, n = 8–10, * p < 0.05, ** p < 0.01, *** p < 0.001. (C) Visual summary of the VEGF-A isoforms and CAP2 markers expression during human prenatal lung development. Image created by Biorender.com .
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    Image Search Results


    Expression of CAP markers and VEGF-A isoforms during human prenatal lung development. RT-qPCR analysis of the expression of universal capillary marker CA4 and CAP2 specific markers ( SOSTDC1 , HPGD, EDNRB, CA4, APLN, and TBX2 ) (A) and of total VEGF-A ( VEGF-Atot ) as well as each VEGF-A isoform ( VEGF-A121 , VEGF-A145 , VEGF-A165 , VEGF-A189 ) (B) in native human prenatal lungs aged from the late pseudoglandular to the early canalicular stages of development (week 10–20 of GA). Results are shown as a dot plot with mean ± SEM, n = 8–10, * p < 0.05, ** p < 0.01, *** p < 0.001. (C) Visual summary of the VEGF-A isoforms and CAP2 markers expression during human prenatal lung development. Image created by Biorender.com .

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: VEGF-A isoforms induce the expression of APLN in endothelial cells during human prenatal lung development

    doi: 10.3389/fcell.2025.1729884

    Figure Lengend Snippet: Expression of CAP markers and VEGF-A isoforms during human prenatal lung development. RT-qPCR analysis of the expression of universal capillary marker CA4 and CAP2 specific markers ( SOSTDC1 , HPGD, EDNRB, CA4, APLN, and TBX2 ) (A) and of total VEGF-A ( VEGF-Atot ) as well as each VEGF-A isoform ( VEGF-A121 , VEGF-A145 , VEGF-A165 , VEGF-A189 ) (B) in native human prenatal lungs aged from the late pseudoglandular to the early canalicular stages of development (week 10–20 of GA). Results are shown as a dot plot with mean ± SEM, n = 8–10, * p < 0.05, ** p < 0.01, *** p < 0.001. (C) Visual summary of the VEGF-A isoforms and CAP2 markers expression during human prenatal lung development. Image created by Biorender.com .

    Article Snippet: The rhVEGF-A isoforms come from R&D system (VEGF-A121: cat#4644-VS-010, VEGF-A145: cat#7626-VE-010, VEGF-A165: cat#293-VE-010, VEGF-A189: cat#8147-VE-025).

    Techniques: Expressing, Quantitative RT-PCR, Marker

    Effect of VEGF-A isoforms on human prenatal lung explant morphology. Representative pictures of the human prenatal lung explant cultures, either untreated or treated with the different VEGF-A isoforms alone or in combinations (VEGF-A121, VEGF-A145, VEGF-A165, VEGF-A189, VEGF-A121-145, and VEGF-A121-145–189) for 48 h. Photos were taken at time 0 (left, (A) and at 48 h (right, (B) . At 48 h, hematoxylin-eosin staining was performed to evaluate the histology of the explants (C) . Scale bar indicated by 50 µm.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: VEGF-A isoforms induce the expression of APLN in endothelial cells during human prenatal lung development

    doi: 10.3389/fcell.2025.1729884

    Figure Lengend Snippet: Effect of VEGF-A isoforms on human prenatal lung explant morphology. Representative pictures of the human prenatal lung explant cultures, either untreated or treated with the different VEGF-A isoforms alone or in combinations (VEGF-A121, VEGF-A145, VEGF-A165, VEGF-A189, VEGF-A121-145, and VEGF-A121-145–189) for 48 h. Photos were taken at time 0 (left, (A) and at 48 h (right, (B) . At 48 h, hematoxylin-eosin staining was performed to evaluate the histology of the explants (C) . Scale bar indicated by 50 µm.

    Article Snippet: The rhVEGF-A isoforms come from R&D system (VEGF-A121: cat#4644-VS-010, VEGF-A145: cat#7626-VE-010, VEGF-A165: cat#293-VE-010, VEGF-A189: cat#8147-VE-025).

    Techniques: Staining

    Impact of VEGF-A isoforms on endothelial cell proliferation in human prenatal lung explants. (A–G) Representative pictures of human prenatal lung explants, aged from the late pseudoglandular to the early canalicular stage (16–21 weeks of GA), untreated (A) or treated with VEGF-A121 (B) , VEGF-A145 (C) , VEGF-A165 (D) , VEGF-A189 (E) , VEGF-A121-145 (F) or VEGF-A121-145-189 (G) for 48 h, stained for CD31 (red) and Ki67 (green) by IF. Nuclei were counterstained with DAPI (blue). (H,I) Associated quantification of the number of CD31-positive cells (H) and the percentage of Ki67-CD31double-positive cells (I) . Results are shown as a dot plot with mean ± SEM, n = 3–9. Scale bar indicated by 50 µm.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: VEGF-A isoforms induce the expression of APLN in endothelial cells during human prenatal lung development

    doi: 10.3389/fcell.2025.1729884

    Figure Lengend Snippet: Impact of VEGF-A isoforms on endothelial cell proliferation in human prenatal lung explants. (A–G) Representative pictures of human prenatal lung explants, aged from the late pseudoglandular to the early canalicular stage (16–21 weeks of GA), untreated (A) or treated with VEGF-A121 (B) , VEGF-A145 (C) , VEGF-A165 (D) , VEGF-A189 (E) , VEGF-A121-145 (F) or VEGF-A121-145-189 (G) for 48 h, stained for CD31 (red) and Ki67 (green) by IF. Nuclei were counterstained with DAPI (blue). (H,I) Associated quantification of the number of CD31-positive cells (H) and the percentage of Ki67-CD31double-positive cells (I) . Results are shown as a dot plot with mean ± SEM, n = 3–9. Scale bar indicated by 50 µm.

    Article Snippet: The rhVEGF-A isoforms come from R&D system (VEGF-A121: cat#4644-VS-010, VEGF-A145: cat#7626-VE-010, VEGF-A165: cat#293-VE-010, VEGF-A189: cat#8147-VE-025).

    Techniques: Staining

    Impact of VEGF-A isoforms on the expression of CAP2 markers in human prenatal lung explants. RT-qPCR analysis of the expression of CAP2 markers ( HPGD , EDNRB , CA4, and APLN ) in human prenatal lung explants aged from the late pseudoglandular to the early canalicular stage (16–21 weeks of GA), untreated or treated with the different VEGF-A isoforms alone or in combinations (VEGF-A121, VEGF-A145, VEGF-A165, VEGF-A189, VEGF-A121-145, and VEGF-A121-145–189) for 48 h. Results are shown as a dot plot with mean ± SEM, n = 4–15, * p < 0.05, ** p < 0.01.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: VEGF-A isoforms induce the expression of APLN in endothelial cells during human prenatal lung development

    doi: 10.3389/fcell.2025.1729884

    Figure Lengend Snippet: Impact of VEGF-A isoforms on the expression of CAP2 markers in human prenatal lung explants. RT-qPCR analysis of the expression of CAP2 markers ( HPGD , EDNRB , CA4, and APLN ) in human prenatal lung explants aged from the late pseudoglandular to the early canalicular stage (16–21 weeks of GA), untreated or treated with the different VEGF-A isoforms alone or in combinations (VEGF-A121, VEGF-A145, VEGF-A165, VEGF-A189, VEGF-A121-145, and VEGF-A121-145–189) for 48 h. Results are shown as a dot plot with mean ± SEM, n = 4–15, * p < 0.05, ** p < 0.01.

    Article Snippet: The rhVEGF-A isoforms come from R&D system (VEGF-A121: cat#4644-VS-010, VEGF-A145: cat#7626-VE-010, VEGF-A165: cat#293-VE-010, VEGF-A189: cat#8147-VE-025).

    Techniques: Expressing, Quantitative RT-PCR

    VEGF-A isoforms induce APLN expression in endothelial cells. (A–G) Representative pictures of human prenatal lung explants, aged from the late pseudoglandular to the early canalicular stage (16–21 weeks of GA), untreated (A) or treated with VEGF-A121 (B) , VEGF-A145 (C) , VEGF-A165 (D) , VEGF-A189 (E) , VEGF-A121-145 (F) or VEGF-A121-145-189 (G) for 48 h, stained for CD31 (green) and Claudin-5 (white) by IF, and for APLN (red) by FISH. Nuclei were counterstained with DAPI (blue). (H,I) Associated quantification of the percentage of APLN + cells (H) and the percentage of APLN + CD31 + CLDN5+ cells (I) . Results are shown as a dot plot with mean ± SEM, n = 4, * p < 0.05, ** p < 0.01. Scale bar indicated by 50 µm.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: VEGF-A isoforms induce the expression of APLN in endothelial cells during human prenatal lung development

    doi: 10.3389/fcell.2025.1729884

    Figure Lengend Snippet: VEGF-A isoforms induce APLN expression in endothelial cells. (A–G) Representative pictures of human prenatal lung explants, aged from the late pseudoglandular to the early canalicular stage (16–21 weeks of GA), untreated (A) or treated with VEGF-A121 (B) , VEGF-A145 (C) , VEGF-A165 (D) , VEGF-A189 (E) , VEGF-A121-145 (F) or VEGF-A121-145-189 (G) for 48 h, stained for CD31 (green) and Claudin-5 (white) by IF, and for APLN (red) by FISH. Nuclei were counterstained with DAPI (blue). (H,I) Associated quantification of the percentage of APLN + cells (H) and the percentage of APLN + CD31 + CLDN5+ cells (I) . Results are shown as a dot plot with mean ± SEM, n = 4, * p < 0.05, ** p < 0.01. Scale bar indicated by 50 µm.

    Article Snippet: The rhVEGF-A isoforms come from R&D system (VEGF-A121: cat#4644-VS-010, VEGF-A145: cat#7626-VE-010, VEGF-A165: cat#293-VE-010, VEGF-A189: cat#8147-VE-025).

    Techniques: Expressing, Staining